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Created on : 24 Dec 2008
Modified on : 29 Oct 2018

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Bibliographical entry (without author) :

Cell-free fetal DNA is not present in plasma of nonpregnant mothers. Clin Chem 50:679-681 and author reply, p 681.

Author(s) :

Rijnders RJ, Christiaens GC, Soussan AA, van der Schoot CE

Year of publication :

2004

URL(s) :

http://clinchem.aaccjnls.org/content/50/3/679.shor…
https://doi.org/10.1373/clinchem.2003.023358

Résumé (français)  :

Abstract (English)  :

Fetal DNA sequences are present in the plasma of pregnant women (1) and can be studied to determine fetal sex(1) and RhD type (2). Unlike fetal cells, fetal cell-free DNA is cleared rapidly from plasma after delivery (3), with a half-life of 16–28 min. Even when clearance is slowed, as in preeclampsia (4), the mean half-life for clearance of fetal DNA is 114 min (5).

Recently, Invernizzi et al. (6) reported positive PCR results for a Y-chromosome-specific sequence in 36 of 160 (22%) women who had given birth to a son up to several years previously. By contrast, other published studies on pregnant women have described no false-positive results (1)(2)(7)(8)(9)(10)(11)(12)(13)(14). It is conceivable that fetal cells remain and proliferate in the maternal circulation or are engrafted in maternal organs after delivery and that this proliferation is suppressed again in a subsequent pregnancy. Lambert et al. (15) also recently reported the presence of fetal DNA in plasma of nonpregnant women. They also found male DNA sequences in 8 of 22 (36%) healthy nonpregnant women who had previously given birth to sons, but filtration studies showed that this DNA was not cell free.

(Ctd. full text)

Sumário (português)  :

Resumen (español)  :

Full text (public) :

Comments :

Argument (français) :

Compte tenu de la détection possible dans le plasma de l’ADN fœtal provenant de cellules apoptotiques, nous recommandons fortement la centrifugation ou la filtration du plasma maternel avant de procéder à un test de diagnostic génétique afin de réduire le risque possible de résultats faussement positifs.

Argument (English):

In view of the possible detection in plasma of fetal DNA from apoptotic cells, we strongly advise centrifugation or filtration of maternal plasma before testing for genetic diagnosis to reduce the possible risk of false-positive results.

Argumento (português):

Em vista da possível detecção no plasma de DNA fetal de células apoptóticas, aconselhamos fortemente a centrifugação ou filtração do plasma materno antes do teste para diagnóstico genético para reduzir o possível risco de resultados falso-positivos.

Argumento (español):

Keywords :

➡ antenatal diagnosis

Author of this record :

Bernard Bel — 24 Dec 2008
➡ latest update : Bernard Bel — 29 Oct 2018