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Criado em : 24 Dec 2008
Alterado em : 29 Oct 2018

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Nota bibliográfica (sem autor) :

Increased fetal DNA concentrations in the plasma of pregnant women carrying fetuses with trisomy 21. Clin Chem 45:1747–1751.

Autores :

Lo YM, et al.

Ano de publicação :

1999

URL(s) :

https://www.ncbi.nlm.nih.gov/pubmed/10508120

Résumé (français)  :

Abstract (English)  :

Background: The recent discovery of the presence of circulating cell-free fetal DNA in maternal plasma opens up new prenatal diagnostic applications and provides new avenues for clinical investigation. It is of research and potential diagnostic interest to determine whether fetal trisomy 21 may be associated with quantitative abnormalities of circulating fetal DNA in maternal plasma.

Methods: Maternal plasma samples were prospectively collected from two centers situated in Hong Kong and Boston. Samples collected from Boston consisted of 7 women carrying male trisomy 21 fetuses, 19 carrying euploid male fetuses, and 13 carrying female fetuses. Samples collected from Hong Kong consisted of 6 women carrying male trisomy 21 fetuses, 18 carrying euploid male fetuses, and 10 carrying female fetuses. Male fetal DNA in maternal plasma was measured using real-time quantitative Y-chromosomal PCR.

Results: For patients recruited from Boston, the median circulating fetal DNA concentrations in women carrying trisomy 21 and euploid male fetuses were 46.0 genome-equivalents/mL and 23.3 genome-equivalents/mL, respectively (P = 0.028). For patients recruited from Hong Kong, the median circulating fetal DNA concentrations in women carrying trisomy 21 and euploid male fetuses were 48.2 genome-equivalents/mL and 16.3 genome-equivalents/mL, respectively (P = 0.026). None of the samples from women carrying female fetuses had detectable Y-chromosomal signals.

Conclusions: Abnormally high concentrations of circulating fetal DNA are found in a proportion of women carrying fetuses with trisomy 21. The robustness and reproducibility of real-time PCR analysis of maternal plasma makes it a valuable tool for cross-institutional collaboration involving centers located in different parts of the world.

Sumário (português)  :

Resumen (español)  :

Texto completo (public) :

Comentários :

Argument (français) :

La robustesse et la reproductibilité de l’analyse PCR en temps réel du plasma maternel en font un outil précieux pour la collaboration interinstitutionnelle impliquant des centres situés dans différentes régions du monde.

Argument (English):

The robustness and reproducibility of real-time PCR analysis of maternal plasma makes it a valuable tool for cross-institutional collaboration involving centers located in different parts of the world.

Argumento (português):

A robustez e a reprodutibilidade da análise PCR em tempo real do plasma materno torna-o uma ferramenta valiosa para a colaboração interinstitucional envolvendo centros localizados em diferentes partes do mundo.

Argumento (español):

Palavras-chaves :

➡ diagnóstico pré-natal

Autor da esta ficha :

Bernard Bel — 24 Dec 2008
➡ última atualização : Bernard Bel — 29 Oct 2018